Australia forward Andrew Nabbout has been ruled out of the World Cup after sustaining a serious shoulder injury during the 1-1 draw with Denmark on Thursday, coach Bert van Marwijk said.

Nabbout grabbed at his shoulder after falling in a challenge and came off grimacing in the 74th minute of the Group C match in Samara.

"He has bad shoulder injury and his tournament is over," Dutchman Van Marwijk told a news conference.

With one point from their opening two games, Australia's chances of reaching the knockout rounds are on a knife edge, and they need victory against Peru in their final match to have any hope of advancing.

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